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genome wide crispr cas9 knockout library screening  (Addgene inc)


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    Addgene inc genome wide crispr cas9 knockout library screening
    Genome Wide Crispr Cas9 Knockout Library Screening, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genome+wide+crispr+cas9+knockout+screen/Human+Genome-wide+CRISPRi-v2+Libraries+(Pooled+Libraries+%2383969%2C+%231000000090)/pm40748748-305-0-11
    Average 95 stars, based on 97 article reviews
    genome wide crispr cas9 knockout library screening - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Genome Wide:

    Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma
    Article Snippet: .. For the genome-wide CRISPR/Cas9 knockout screen, the A673/EWSR1::FLI1 tdTomato /EGFP/Cas9 reporter cell line was transduced with the Human CRISPR Knockout Pooled Library (Brunello) (Addgene, Cat# 73178) , consisting of ~76,441 sgRNAs targeting 19,114 genes at multiplicity-of-infection (MOI) of 0.3. ..

    Article Title: SAYSD1 senses UFMylated ribosome to safeguard co-translational protein translocation at the endoplasmic reticulum.
    Article Snippet: .. Genome-wide CRISPR/Cas9 knockout screen The GeckoV2 library was purchased from Addgene (1,000,000,048) and amplified according to the Addgene’s protocol and the online protocol from Feng Zhang’s lab.25 The complexity of the sgRNA library was verified by high-throughput sequencing by the NIDDK Genomic Core. ..

    Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma.
    Article Snippet: .. For the genome-wide CRISPR/Cas9 knockout screen, the A673/ EWSR1::FLI1tdTomato/EGFP/Cas9 reporter cell line was transduced with the Human CRISPR Knockout Pooled Library (Brunello) (Addgene, Cat# 73178)10, consisting of ~76,441 sgRNAs targeting 19,114 genes at multiplicity-of-infection (MOI) of 0.3. ..

    Article Title: Uridine-cytidine kinase 2 potentiates the mutagenic influence of the antiviral β-d-N4-hydroxycytidine.
    Article Snippet: .. Genome-wide CRISPR / Cas9 knockout screen The Genome-wide Mouse Lentiviral CRISPR gRNA Library v1 was used for our screen ( Addgene, Pooled Library #50947 ) ( 18 ) . .. To prepare the lentivirus, 1.5 μg of the library plasmid was used for co-transfection with packaging plasmids ( 1 μg pCMV-Rev ( Addgene 115776 ) , 1.5 μg pMDLg / pRRE ( Addgene 12251 ) , 1 μg pCMV -VSV -G ( Addgene 8454 ) ) for each 10 cm dish of HEK293T using 24 μl FuGENE HD Transfection Reagent ( Promega, E2311 ) .

    Article Title: Glucose-6-phosphate dehydrogenase regulates mitophagy by maintaining PINK1 stability.
    Article Snippet: .. Genome-wide CRISPR/Cas9 knockout screen The Human Two Plasmid Activity-Optimized CRISPR Knockout Library constructed by David Sabatini/Eric Lander and colleagues was obtained from Addgene (#1000000095) [41]. ..

    Article Title: The Bordetella effector protein BteA induces host cell death by disruption of calcium homeostasis.
    Article Snippet: .. Production of lentiviral vector library and genome-wide CRISPR-Cas9 knockout screen in HEK-Cas9 cells The production of lentiviral vector library GeCKO v2 (Addgene item #1000000049 [35]) consisting of sublibraries A and B and preparation of the corresponding sublibraries of HEK-Cas9 cells were done according to the protocol reported by Joung et al. (73). ..

    Article Title: Genome-wide CRISPR screens define determinants of epithelial-mesenchymal transition mediated immune evasion by pancreatic cancer cells.
    Article Snippet: The selective small molecule TβRI/ALK5 kinase inhibitor SB505124 (#3263) was used at 1 mM (#3263, Tocris, Bristol, UK). .. Genome-wide CRISPR-Cas9 knockout screen and analysis The genome-wide Brie CRISPR-knockout (KO) library (four sgRNAs per gene; ~78,637 sgRNAs) was purchased from Addgene (#73632) (32) and amplified according to the supplier’s protocol. ..

    CRISPR:

    Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma
    Article Snippet: .. For the genome-wide CRISPR/Cas9 knockout screen, the A673/EWSR1::FLI1 tdTomato /EGFP/Cas9 reporter cell line was transduced with the Human CRISPR Knockout Pooled Library (Brunello) (Addgene, Cat# 73178) , consisting of ~76,441 sgRNAs targeting 19,114 genes at multiplicity-of-infection (MOI) of 0.3. ..

    Article Title: SAYSD1 senses UFMylated ribosome to safeguard co-translational protein translocation at the endoplasmic reticulum.
    Article Snippet: .. Genome-wide CRISPR/Cas9 knockout screen The GeckoV2 library was purchased from Addgene (1,000,000,048) and amplified according to the Addgene’s protocol and the online protocol from Feng Zhang’s lab.25 The complexity of the sgRNA library was verified by high-throughput sequencing by the NIDDK Genomic Core. ..

    Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma.
    Article Snippet: .. For the genome-wide CRISPR/Cas9 knockout screen, the A673/ EWSR1::FLI1tdTomato/EGFP/Cas9 reporter cell line was transduced with the Human CRISPR Knockout Pooled Library (Brunello) (Addgene, Cat# 73178)10, consisting of ~76,441 sgRNAs targeting 19,114 genes at multiplicity-of-infection (MOI) of 0.3. ..

    Article Title: Uridine-cytidine kinase 2 potentiates the mutagenic influence of the antiviral β-d-N4-hydroxycytidine.
    Article Snippet: .. Genome-wide CRISPR / Cas9 knockout screen The Genome-wide Mouse Lentiviral CRISPR gRNA Library v1 was used for our screen ( Addgene, Pooled Library #50947 ) ( 18 ) . .. To prepare the lentivirus, 1.5 μg of the library plasmid was used for co-transfection with packaging plasmids ( 1 μg pCMV-Rev ( Addgene 115776 ) , 1.5 μg pMDLg / pRRE ( Addgene 12251 ) , 1 μg pCMV -VSV -G ( Addgene 8454 ) ) for each 10 cm dish of HEK293T using 24 μl FuGENE HD Transfection Reagent ( Promega, E2311 ) .

    Article Title: Glucose-6-phosphate dehydrogenase regulates mitophagy by maintaining PINK1 stability.
    Article Snippet: .. Genome-wide CRISPR/Cas9 knockout screen The Human Two Plasmid Activity-Optimized CRISPR Knockout Library constructed by David Sabatini/Eric Lander and colleagues was obtained from Addgene (#1000000095) [41]. ..

    Article Title: The Bordetella effector protein BteA induces host cell death by disruption of calcium homeostasis.
    Article Snippet: .. Production of lentiviral vector library and genome-wide CRISPR-Cas9 knockout screen in HEK-Cas9 cells The production of lentiviral vector library GeCKO v2 (Addgene item #1000000049 [35]) consisting of sublibraries A and B and preparation of the corresponding sublibraries of HEK-Cas9 cells were done according to the protocol reported by Joung et al. (73). ..

    Article Title: Genome-wide CRISPR screens define determinants of epithelial-mesenchymal transition mediated immune evasion by pancreatic cancer cells.
    Article Snippet: The selective small molecule TβRI/ALK5 kinase inhibitor SB505124 (#3263) was used at 1 mM (#3263, Tocris, Bristol, UK). .. Genome-wide CRISPR-Cas9 knockout screen and analysis The genome-wide Brie CRISPR-knockout (KO) library (four sgRNAs per gene; ~78,637 sgRNAs) was purchased from Addgene (#73632) (32) and amplified according to the supplier’s protocol. ..

    Knock-Out:

    Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma
    Article Snippet: .. For the genome-wide CRISPR/Cas9 knockout screen, the A673/EWSR1::FLI1 tdTomato /EGFP/Cas9 reporter cell line was transduced with the Human CRISPR Knockout Pooled Library (Brunello) (Addgene, Cat# 73178) , consisting of ~76,441 sgRNAs targeting 19,114 genes at multiplicity-of-infection (MOI) of 0.3. ..

    Article Title: SAYSD1 senses UFMylated ribosome to safeguard co-translational protein translocation at the endoplasmic reticulum.
    Article Snippet: .. Genome-wide CRISPR/Cas9 knockout screen The GeckoV2 library was purchased from Addgene (1,000,000,048) and amplified according to the Addgene’s protocol and the online protocol from Feng Zhang’s lab.25 The complexity of the sgRNA library was verified by high-throughput sequencing by the NIDDK Genomic Core. ..

    Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma.
    Article Snippet: .. For the genome-wide CRISPR/Cas9 knockout screen, the A673/ EWSR1::FLI1tdTomato/EGFP/Cas9 reporter cell line was transduced with the Human CRISPR Knockout Pooled Library (Brunello) (Addgene, Cat# 73178)10, consisting of ~76,441 sgRNAs targeting 19,114 genes at multiplicity-of-infection (MOI) of 0.3. ..

    Article Title: Uridine-cytidine kinase 2 potentiates the mutagenic influence of the antiviral β-d-N4-hydroxycytidine.
    Article Snippet: .. Genome-wide CRISPR / Cas9 knockout screen The Genome-wide Mouse Lentiviral CRISPR gRNA Library v1 was used for our screen ( Addgene, Pooled Library #50947 ) ( 18 ) . .. To prepare the lentivirus, 1.5 μg of the library plasmid was used for co-transfection with packaging plasmids ( 1 μg pCMV-Rev ( Addgene 115776 ) , 1.5 μg pMDLg / pRRE ( Addgene 12251 ) , 1 μg pCMV -VSV -G ( Addgene 8454 ) ) for each 10 cm dish of HEK293T using 24 μl FuGENE HD Transfection Reagent ( Promega, E2311 ) .

    Article Title: Glucose-6-phosphate dehydrogenase regulates mitophagy by maintaining PINK1 stability.
    Article Snippet: .. Genome-wide CRISPR/Cas9 knockout screen The Human Two Plasmid Activity-Optimized CRISPR Knockout Library constructed by David Sabatini/Eric Lander and colleagues was obtained from Addgene (#1000000095) [41]. ..

    Article Title: The Bordetella effector protein BteA induces host cell death by disruption of calcium homeostasis.
    Article Snippet: .. Production of lentiviral vector library and genome-wide CRISPR-Cas9 knockout screen in HEK-Cas9 cells The production of lentiviral vector library GeCKO v2 (Addgene item #1000000049 [35]) consisting of sublibraries A and B and preparation of the corresponding sublibraries of HEK-Cas9 cells were done according to the protocol reported by Joung et al. (73). ..

    Article Title: Genome-wide CRISPR screens define determinants of epithelial-mesenchymal transition mediated immune evasion by pancreatic cancer cells.
    Article Snippet: The selective small molecule TβRI/ALK5 kinase inhibitor SB505124 (#3263) was used at 1 mM (#3263, Tocris, Bristol, UK). .. Genome-wide CRISPR-Cas9 knockout screen and analysis The genome-wide Brie CRISPR-knockout (KO) library (four sgRNAs per gene; ~78,637 sgRNAs) was purchased from Addgene (#73632) (32) and amplified according to the supplier’s protocol. ..

    Transduction:

    Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma
    Article Snippet: .. For the genome-wide CRISPR/Cas9 knockout screen, the A673/EWSR1::FLI1 tdTomato /EGFP/Cas9 reporter cell line was transduced with the Human CRISPR Knockout Pooled Library (Brunello) (Addgene, Cat# 73178) , consisting of ~76,441 sgRNAs targeting 19,114 genes at multiplicity-of-infection (MOI) of 0.3. ..

    Article Title: The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcoma.
    Article Snippet: .. For the genome-wide CRISPR/Cas9 knockout screen, the A673/ EWSR1::FLI1tdTomato/EGFP/Cas9 reporter cell line was transduced with the Human CRISPR Knockout Pooled Library (Brunello) (Addgene, Cat# 73178)10, consisting of ~76,441 sgRNAs targeting 19,114 genes at multiplicity-of-infection (MOI) of 0.3. ..

    Amplification:

    Article Title: SAYSD1 senses UFMylated ribosome to safeguard co-translational protein translocation at the endoplasmic reticulum.
    Article Snippet: .. Genome-wide CRISPR/Cas9 knockout screen The GeckoV2 library was purchased from Addgene (1,000,000,048) and amplified according to the Addgene’s protocol and the online protocol from Feng Zhang’s lab.25 The complexity of the sgRNA library was verified by high-throughput sequencing by the NIDDK Genomic Core. ..

    Article Title: Genome-wide CRISPR screens define determinants of epithelial-mesenchymal transition mediated immune evasion by pancreatic cancer cells.
    Article Snippet: The selective small molecule TβRI/ALK5 kinase inhibitor SB505124 (#3263) was used at 1 mM (#3263, Tocris, Bristol, UK). .. Genome-wide CRISPR-Cas9 knockout screen and analysis The genome-wide Brie CRISPR-knockout (KO) library (four sgRNAs per gene; ~78,637 sgRNAs) was purchased from Addgene (#73632) (32) and amplified according to the supplier’s protocol. ..

    Next-Generation Sequencing:

    Article Title: SAYSD1 senses UFMylated ribosome to safeguard co-translational protein translocation at the endoplasmic reticulum.
    Article Snippet: .. Genome-wide CRISPR/Cas9 knockout screen The GeckoV2 library was purchased from Addgene (1,000,000,048) and amplified according to the Addgene’s protocol and the online protocol from Feng Zhang’s lab.25 The complexity of the sgRNA library was verified by high-throughput sequencing by the NIDDK Genomic Core. ..

    Plasmid Preparation:

    Article Title: Glucose-6-phosphate dehydrogenase regulates mitophagy by maintaining PINK1 stability.
    Article Snippet: .. Genome-wide CRISPR/Cas9 knockout screen The Human Two Plasmid Activity-Optimized CRISPR Knockout Library constructed by David Sabatini/Eric Lander and colleagues was obtained from Addgene (#1000000095) [41]. ..

    Article Title: The Bordetella effector protein BteA induces host cell death by disruption of calcium homeostasis.
    Article Snippet: .. Production of lentiviral vector library and genome-wide CRISPR-Cas9 knockout screen in HEK-Cas9 cells The production of lentiviral vector library GeCKO v2 (Addgene item #1000000049 [35]) consisting of sublibraries A and B and preparation of the corresponding sublibraries of HEK-Cas9 cells were done according to the protocol reported by Joung et al. (73). ..

    Activity Assay:

    Article Title: Glucose-6-phosphate dehydrogenase regulates mitophagy by maintaining PINK1 stability.
    Article Snippet: .. Genome-wide CRISPR/Cas9 knockout screen The Human Two Plasmid Activity-Optimized CRISPR Knockout Library constructed by David Sabatini/Eric Lander and colleagues was obtained from Addgene (#1000000095) [41]. ..

    Construct:

    Article Title: Glucose-6-phosphate dehydrogenase regulates mitophagy by maintaining PINK1 stability.
    Article Snippet: .. Genome-wide CRISPR/Cas9 knockout screen The Human Two Plasmid Activity-Optimized CRISPR Knockout Library constructed by David Sabatini/Eric Lander and colleagues was obtained from Addgene (#1000000095) [41]. ..



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    Image Search Results


    Journal: Cell reports

    Article Title: SMYD5 is a regulator of the mild hypothermia response

    doi: 10.1016/j.celrep.2024.114554

    Figure Lengend Snippet:

    Article Snippet: Using the 293WT + Cas9+SP1 and 293WT + Cas9 (negative control) cell lines we performed a genome wide CRISPR-Cas9 knockout screen (GeCKO) screen, where we transduced the sgRNA pool made from library A and B (Addgene, #1000000049) in MOI 0.3.

    Techniques: Mutagenesis, Inhibition, Reverse Transcription, BIA-KA, Sample Prep, CRISPR, Recombinant, Plasmid Preparation, Software